Difference between revisions of "Part:BBa K2100013"
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<partinfo>BBa_K2100013 short</partinfo> | <partinfo>BBa_K2100013 short</partinfo> | ||
− | tagBFP is a blue fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter. | + | tagBFP is a blue fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter. This cascade can be used as a transfection marker to determine your efficiency for many experiments in mammalian synbio. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 03:58, 23 October 2016
pEXPR hEF1a-tagBFP
tagBFP is a blue fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter. This cascade can be used as a transfection marker to determine your efficiency for many experiments in mammalian synbio.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal BglII site found at 591
Illegal BamHI site found at 1197
Illegal BamHI site found at 1227
Illegal XhoI site found at 990 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842
Illegal NgoMIV site found at 725
Illegal AgeI site found at 103 - 1000COMPATIBLE WITH RFC[1000]