Difference between revisions of "Part:BBa K2066029:Design"
(→Design Notes) |
(→Source) |
||
| Line 11: | Line 11: | ||
===Source=== | ===Source=== | ||
| + | Linker and mCherry taken from Synthetic Analog Computation in Living Cells (Daniel et al.). TetR from NCBI NC_011812.1 | ||
===References=== | ===References=== | ||
Latest revision as of 03:04, 19 October 2016
pBad - TetR/mCherry Fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1245
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1184
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1019
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1001
Design Notes
Codons were optimized for E. Coli expression and non compatible restriction sites were removed.
Source
Linker and mCherry taken from Synthetic Analog Computation in Living Cells (Daniel et al.). TetR from NCBI NC_011812.1