Difference between revisions of "Part:BBa K2040121:Design"

Latest revision as of 09:18, 19 October 2016

PgpdA + KillerRed + TtrpC

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 880
Illegal BsaI.rc site found at 1171

Design Notes

KillerRed can be replaced by KillerRed-NLS so that KillerRed can be localized in the nucleus to increase efficiency of KillerRed-mediated oxidative stress.

Two illegal cutsites (EcoRI and XbaI) were subsequently removed via site-directed mutagenesis.

This device doesn't include a RBS because it's just for fungal expression.

Source

KillerRed was engineered from anm2CP.1^[1]

PgpdA,TtrpC ,from Aspergillus nidulans, were isolated from vector pBARGPE1.

References

[1]Bulina, Maria E, Chudakov, Dmitriy M, Britanova, Olga V, Yanushevich, Yurii G, Staroverov, Dmitry B, Chepurnykh, Tatyana V, Merzlyak, Ekaterina M, Shkrob, Maria A, Lukyanov, Sergey, Lukyanov and Konstantin A.
A Genetically Encoded Photosensitizer
Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175

@@ Line 7: / Line 7: @@
 ===Design Notes===
-KillerRed can be replaced by KillerRed-NLS so that KillerRed can be localized in the nucleus.
+KillerRed can be replaced by KillerRed-NLS so that KillerRed can be localized in the nucleus to increase efficiency of KillerRed-mediated oxidative stress.
+Two illegal cutsites (EcoRI and XbaI) were subsequently removed via site-directed mutagenesis.
+This device doesn't include a RBS because it's just for fungal expression.
 ===Source===
-KillerRed: Engineered from anm2CP.1<sup>[1]</sup>
+KillerRed was engineered from anm2CP.1<sup>[1]</sup>
-PgpdA,TtrpC were from ''Aspergillus nidulans''.
+PgpdA,TtrpC ,from ''Aspergillus nidulans'', were isolated from vector pBARGPE1.
 ===References===