Difference between revisions of "Part:BBa K2040121:Design"

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===Design Notes===
 
===Design Notes===
−
KillerRed can be replaced by KillerRed-NLS so that KillerRed can be localized in the nucleus.
+
KillerRed can be replaced by KillerRed-NLS so that KillerRed can be localized in the nucleus to increase efficiency of KillerRed-mediated oxidative stress.
  
 +
Two illegal cutsites (EcoRI and XbaI) were subsequently removed via site-directed mutagenesis.
  
 +
This device doesn't include a RBS because it's just for fungal expression.
  
 
===Source===
 
===Source===
  
−
KillerRed: Engineered from anm2CP.1<sup>[1]</sup>
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KillerRed was engineered from anm2CP.1<sup>[1]</sup>
−
PgpdA was from ''Aspergillus nidulans''.
+
 
 +
PgpdA,TtrpC ,from ''Aspergillus nidulans'', were isolated from vector pBARGPE1.
  
 
===References===
 
===References===
 +
[1]Bulina, Maria E, Chudakov, Dmitriy M, Britanova, Olga V, Yanushevich, Yurii G, Staroverov, Dmitry B, Chepurnykh, Tatyana V, Merzlyak, Ekaterina M, Shkrob, Maria A, Lukyanov, Sergey, Lukyanov and Konstantin A. <br />
 +
<i>A Genetically Encoded Photosensitizer</i><br />
 +
Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175

Latest revision as of 09:18, 19 October 2016


PgpdA + KillerRed + TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 880
    Illegal BsaI.rc site found at 1171


Design Notes

KillerRed can be replaced by KillerRed-NLS so that KillerRed can be localized in the nucleus to increase efficiency of KillerRed-mediated oxidative stress.

Two illegal cutsites (EcoRI and XbaI) were subsequently removed via site-directed mutagenesis.

This device doesn't include a RBS because it's just for fungal expression.

Source

KillerRed was engineered from anm2CP.1[1]

PgpdA,TtrpC ,from Aspergillus nidulans, were isolated from vector pBARGPE1.

References

[1]Bulina, Maria E, Chudakov, Dmitriy M, Britanova, Olga V, Yanushevich, Yurii G, Staroverov, Dmitry B, Chepurnykh, Tatyana V, Merzlyak, Ekaterina M, Shkrob, Maria A, Lukyanov, Sergey, Lukyanov and Konstantin A.
A Genetically Encoded Photosensitizer
Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175