Difference between revisions of "Part:BBa K2150112"
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We measured the growth curve of bacteria expressing toxin N-acetyltransferase GCN5. | We measured the growth curve of bacteria expressing toxin N-acetyltransferase GCN5. | ||
| − | [[file:T--UCAS-- | + | [[file:T--UCAS--1204t-atc.png|500px]] |
The growth curve of wild type E. coli harboring empty vector is shown below. | The growth curve of wild type E. coli harboring empty vector is shown below. | ||
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[[file:T--UCAS--RGP_Ptet.png|500px]] | [[file:T--UCAS--RGP_Ptet.png|500px]] | ||
| + | Wee also studied the interaction between toxin and antitoxin. See: | ||
| + | [https://parts.igem.org/Part:BBa_K2150116 BBa_K2150116] | ||
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Latest revision as of 04:04, 20 October 2016
Contents
toxin N-acetyltransferase GCN5 induced by aTc
Usage and Biology
This part is used to build a kill-switch.
Characterization
Growth Curve
We measured the growth curve of bacteria expressing toxin N-acetyltransferase GCN5.
The growth curve of wild type E. coli harboring empty vector is shown below.
Wee also studied the interaction between toxin and antitoxin. See: BBa_K2150116
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 240
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 486
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 240
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 240
Illegal NgoMIV site found at 417 - 1000COMPATIBLE WITH RFC[1000]