Difference between revisions of "Part:BBa K2150111"
 
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[[file:T--UCAS--RGP_Ptac.png|500px]]
 
[[file:T--UCAS--RGP_Ptac.png|500px]]
  
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We also studied the interaction between ParE2 and its corresponding antitoxin ParD2. See:
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[https://parts.igem.org/Part:BBa_K2150117 BBa_K2150117]
  
 
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Latest revision as of 03:43, 20 October 2016

toxin ParE2 induced by IPTG

Usage and Biology

This part is used to build a kill-switch.

Characterization

Growth Curve

We measured the growth curve of bacteria expressing toxin ParE2.

T--UCAS--6249t-IPTG.png

The growth curve of wild type E. coli harboring empty vector is shown below.

T--UCAS--RGP Ptac.png

We also studied the interaction between ParE2 and its corresponding antitoxin ParD2. See: BBa_K2150117

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1848
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]