Difference between revisions of "Part:BBa K733004:Experience"

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===Applications of BBa_K733004===
 
===Applications of BBa_K733004===
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<h3>UCAS 2016</h3>
 
We are iGEM team from University of Chinese Academy of Sciences.
 
We are iGEM team from University of Chinese Academy of Sciences.
 
In this year's program, we used this part to build a kill-switch.
 
In this year's program, we used this part to build a kill-switch.
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When identifying interaction between toxin and antitoxin, toxin is induced by aTc.
 
When identifying interaction between toxin and antitoxin, toxin is induced by aTc.
  
<h3>Growth curve E. coli expressing toxin EndoA</h3>
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<h4>Growth curve of E. coli expressing toxin EndoA</h4>
  
[[file:T--UCAS--134-toxin-IPTG.tif]]
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[[file:T--UCAS--134-toxin-IPTG1.png|500px]]
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We also measured the growth curve of wild type E. coli harboring empty plasmids.
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[[file:T--UCAS--RGP_Ptac.png|500px]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 01:58, 19 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K733004

UCAS 2016

We are iGEM team from University of Chinese Academy of Sciences. In this year's program, we used this part to build a kill-switch. The gene is induced by IPTG or aTc. When identifying interaction between toxin and antitoxin, toxin is induced by aTc.

Growth curve of E. coli expressing toxin EndoA

T--UCAS--134-toxin-IPTG1.png

We also measured the growth curve of wild type E. coli harboring empty plasmids.

T--UCAS--RGP Ptac.png

User Reviews

UNIQ09f0efc6b8a58077-partinfo-00000000-QINU UNIQ09f0efc6b8a58077-partinfo-00000001-QINU