Difference between revisions of "Part:BBa K2065000:Experience"
Tjvsonsbeek (Talk | contribs) (→iGEM TU Eindhoven 2016) |
Tjvsonsbeek (Talk | contribs) (→iGEM TU Eindhoven 2016) |
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==iGEM TU Eindhoven 2016== | ==iGEM TU Eindhoven 2016== | ||
| − | iGEM TU Eindhoven used this part in combination with CT52-LargeBiT (BBa_K2065007). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. | + | iGEM TU Eindhoven used this part in combination with CT52-LargeBiT ("https://parts.igem.org/Part:BBa_K2065007"). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. This system was used as a read out method for T14-3-3 heterodimers. It is schemtically visualised in figure 1. For more detailed information about the application of CT52-SmallBiT visit our wiki "http://2016.igem.org/Team:TU-Eindhoven/Read-out". |
[[File:T--TU-Eindhoven--NanolUcreadoutbb.png]] | [[File:T--TU-Eindhoven--NanolUcreadoutbb.png]] | ||
<br> | <br> | ||
| − | ''Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52'' | + | ''Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52'' |
Latest revision as of 00:19, 19 October 2016
Applications of BBa_K2065000
iGEM TU Eindhoven 2016
iGEM TU Eindhoven used this part in combination with CT52-LargeBiT ("https://parts.igem.org/Part:BBa_K2065007"). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. This system was used as a read out method for T14-3-3 heterodimers. It is schemtically visualised in figure 1. For more detailed information about the application of CT52-SmallBiT visit our wiki "http://2016.igem.org/Team:TU-Eindhoven/Read-out".
Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52
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