Difference between revisions of "Part:BBa K1941001"
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| − | A scaffold RNA is a guide RNA (gRNA), supplemented with an additional hairpin that can recruit different RNA binding module. In | + | A scaffold RNA is a guide RNA (gRNA), supplemented with an additional hairpin that can recruit different RNA binding module. In this case, the hairpin is PP7, a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter TEF1. |
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Latest revision as of 23:04, 18 October 2016
scTef1_PP7
Scaffold RNA (scRNA) with PP7 hairpin that targets TEF1 promoter.
Usage and Biology
A scaffold RNA is a guide RNA (gRNA), supplemented with an additional hairpin that can recruit different RNA binding module. In this case, the hairpin is PP7, a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter TEF1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]