Difference between revisions of "Part:BBa K1941000:Design"

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===Design Notes===
 
===Design Notes===
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Mxi1 was fused to PCP based on the fusion between VP64, a transcriptional activator, and MCP, another RNA binding module described in "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds", J.G. Zalatan, Cell 2015.
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The link between PCP and Mxi1 is 6 base pairs long.
  
 
===Source===
 
===Source===

Latest revision as of 20:35, 18 October 2016


PCP fused to Mxi1 domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 333
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mxi1 was fused to PCP based on the fusion between VP64, a transcriptional activator, and MCP, another RNA binding module described in "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds", J.G. Zalatan, Cell 2015. The link between PCP and Mxi1 is 6 base pairs long.

Source

PCP sequence taken from "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds", J.G. Zalatan, Cell 2015

References