Difference between revisions of "Part:BBa K1893006"
 
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<partinfo>BBa_K1893006 short</partinfo>
 
<partinfo>BBa_K1893006 short</partinfo>
  
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The Cin receiver device consists of a constitutively expressed CinR transcription regulator [https://parts.igem.org/Part:BBa_K1893028 (BBa_K1893028)] upstream of a cognate pCin promoter [https://parts.igem.org/Part:BBa_K1893029 (BBa_K1893029)]. CinR is activated in the presence of a O3-C14 HSL signal and binds to pCin to initiate transcription of a downstream coding sequence.
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===Usage and biology===
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The Cin receiver is based on the Cin quorum-sensing system from <i>Rhizobium leguminosarum</i>. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
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In this case, the constitutively expressed CinR transcriptional regulator [https://parts.igem.org/Part:BBa_K1893028 (BBa_K1893028)] is activated by the binding of O3-C14 HSL, an AHL quorum signal. The activated CinR regulator binds to the CinR-inducible promoter, pCin, to initiate transcription of a downstream gene.
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===Characterisation===
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We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here [https://parts.igem.org/Part:BBa_K1893007 (BBa_K1893007)]
  
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The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. CinR is activated by 3OC14-HSL. Included in the construct is the CinR-activated promoter pCin.
 
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1893006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1893006 SequenceAndFeatures</partinfo>

Latest revision as of 03:20, 29 October 2016


Cin receiver (CinR+pCin)

The Cin receiver device consists of a constitutively expressed CinR transcription regulator (BBa_K1893028) upstream of a cognate pCin promoter (BBa_K1893029). CinR is activated in the presence of a O3-C14 HSL signal and binds to pCin to initiate transcription of a downstream coding sequence.

Usage and biology

The Cin receiver is based on the Cin quorum-sensing system from Rhizobium leguminosarum. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.

In this case, the constitutively expressed CinR transcriptional regulator (BBa_K1893028) is activated by the binding of O3-C14 HSL, an AHL quorum signal. The activated CinR regulator binds to the CinR-inducible promoter, pCin, to initiate transcription of a downstream gene.

Characterisation

We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here (BBa_K1893007)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 611
  • 1000
    COMPATIBLE WITH RFC[1000]