Difference between revisions of "Part:BBa K2065001:Experience"
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==iGEM TU Eindhoven 2016==
 
==iGEM TU Eindhoven 2016==
  
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We succeeded in creating the construct at molecular level. Unfortunately, there was not enough time left to express the protein, neither to do assays with it.
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We succeeded in creating this construct. Because the construct which on which mNeonGreen was used did finish just too late to do in vitro assays with, namely a BRET assay. For this BRET assay, the CT52-mNeongreen as well as CT52-LargeBit and CT52-SmallBit were assembled on the T14-3-3 scaffold protein. CT52-mNeonGreen will be the acceptor that changes the wavelength of the light emitted by NanoLuc to a wavelength of 517 nm. This system is visible in figure 1.
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A few of us will, might continue with this construct in future research.
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[[File:T--TU-Eindhoven--BRETbb.png]]
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''Figure 1: Schematic representaton of the BRET system with tetrameric T14-3-3 scaffold protein and the NanoBiT system linked to CT52''

Latest revision as of 00:25, 19 October 2016


Applications of BBa_K2065000

iGEM TU Eindhoven 2016

We succeeded in creating this construct. Because the construct which on which mNeonGreen was used did finish just too late to do in vitro assays with, namely a BRET assay. For this BRET assay, the CT52-mNeongreen as well as CT52-LargeBit and CT52-SmallBit were assembled on the T14-3-3 scaffold protein. CT52-mNeonGreen will be the acceptor that changes the wavelength of the light emitted by NanoLuc to a wavelength of 517 nm. This system is visible in figure 1.


T--TU-Eindhoven--BRETbb.png
Figure 1: Schematic representaton of the BRET system with tetrameric T14-3-3 scaffold protein and the NanoBiT system linked to CT52