Difference between revisions of "Part:BBa K2120301"
 
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<partinfo>BBa_K2120301 short</partinfo>
 
<partinfo>BBa_K2120301 short</partinfo>
  
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The PR promoter has two DNA binding sites for lambada CI repressor[https://parts.igem.org/wiki/index.php?title=Part:BBa_c0051 BBa_C0051].and RFP is the reporter gene to test the inhibit efficiency of PR promoter. In our project, the reporter gene indicate the strength of promoter. We use it in our validation circuit.
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This device is composed of induced promoter pR , ''cI'' ([https://parts.igem.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051]), and a composite part J133000([https://parts.igem.org/wiki/index.php?title=Part:BBa_J133000 BBa_J133000]). ''RFP'' is employed as report gene. The pR promoter can be repressed by its inhibitor ''cI''. The expression of RFP will decrease corresponding the increasing of inhibitor to indicate the switch’s expression level.
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This device is designed to construct K2120310([https://parts.igem.org/wiki/index.php?title=Part:BBa_K2120310 BBa_K2120310]).
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 15:21, 22 October 2016


pR+B0032+RFP+B0015

This device is composed of induced promoter pR , cI (BBa_C0051), and a composite part J133000(BBa_J133000). RFP is employed as report gene. The pR promoter can be repressed by its inhibitor cI. The expression of RFP will decrease corresponding the increasing of inhibitor to indicate the switch’s expression level. This device is designed to construct K2120310(BBa_K2120310).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 631
    Illegal AgeI site found at 743
  • 1000
    COMPATIBLE WITH RFC[1000]