Difference between revisions of "Part:BBa K2040101:Design"

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===Design Notes===
 
===Design Notes===
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One illegal cutsites (PstI) were subsequently removed via site-directed mutagenesis.
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One illegal cutsite (PstI) were subsequently removed via site-directed mutagenesis.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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NCBI: Expression vector (PAN7-1) DNA    https://www.ncbi.nlm.nih.gov/nuccore/Z32698.1

Latest revision as of 18:04, 18 October 2016


Fungal promoter PgpdA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
    Illegal XhoI site found at 693
    Illegal XhoI site found at 1075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
    Illegal AgeI site found at 1430
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109
    Illegal SapI site found at 315


Design Notes

One illegal cutsite (PstI) were subsequently removed via site-directed mutagenesis.

Source

It was taken from vector pAN7-1.

References

NCBI: Expression vector (PAN7-1) DNA https://www.ncbi.nlm.nih.gov/nuccore/Z32698.1