Difference between revisions of "Part:BBa K1899006"

Latest revision as of 15:37, 18 October 2016

J23101-B0032-lacl-B1006- phlFp-GFP

This construct aims at investigating any interference caused by Lacl repressor on promoter phlFp.

Results

The fold change between construct A (pSB3K3-BBa_E0240-phlFp) and negative control (pSB3K3-BBa_E0240), and that of construct C (pSB3K3-BBa_J23101-B0032-C0012-B1006- phlFp -E0240) were both approximately 13.2. Since their RFU are similar, it indicated that there is no cross-talk between lacp and phlFp (BBa_K1899004).

Fig 1. Comparison on fluorescence expression levels of construct A (BBa_phlFp-E0240) and construct C (BBa_J23101-B0032-C0012-B1006- phlFp -E0240). Negative control represents BBa_E0240. Characterization was done using E. coli strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1209
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2007

@@ Line 6: / Line 6: @@
 ===Results===
-The fold change between construct A (pSB3K3-<i>phlFp</i>- BBa_E0240) and negative control (pSB3K3-BBa_E0240), and that of construct C (pSB3K3-BBa_J23101-B0032-C0012-B1006- <i>phlFp</i> -E0240) were both approximately 13.2. Since their RFU are similar, it indicated that there is no cross-talk between <i>lacp</i> and<i>phlFp</i>(<bbpart>(BBa_K1899004</bbpart>).
+The fold change between construct A (pSB3K3-BBa_E0240-<i>phlFp</i>) and negative control (pSB3K3-BBa_E0240), and that of construct C (pSB3K3-BBa_J23101-B0032-C0012-B1006- <i>phlFp</i> -E0240) were both approximately 13.2. Since their RFU are similar, it indicated that there is no cross-talk between <i>lacp</i> and <i>phlFp</i> (<bbpart>(BBa_K1899004</bbpart>).
 <br>
 [[File:IGEM2016_HKUST_phlFK1899006.png|thumb|600px|center|<b>Fig 1. Comparison on fluorescence expression levels of construct A (BBa_<i>phlFp</i>-E0240) and construct C (BBa_J23101-B0032-C0012-B1006- <i>phlFp</i> -E0240)</b>. Negative control represents BBa_E0240. Characterization was done using <i>E. coli</i> strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.]]