Difference between revisions of "Part:BBa K1898150"
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<partinfo>BBa_K1898150 short</partinfo> | <partinfo>BBa_K1898150 short</partinfo> | ||
| − | + | BBa_K880005 consists of a strong promoter and strong RBS and was used to maximize protein production. This construct codes for CH25H (cholesterol 25-Hydroxylase) and a 10x Histidine-tag. CH25H is a catalyst that will make 25 hydroxycholesterol from cholesterol and 10x Histidine-tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription. | |
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| − | ==Synthesis Report== | + | ===Synthesis Report=== |
This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below: | This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below: | ||
| + | *Prefix and suffix are highlighted in orange | ||
| + | *BBa_K880005 is highlighted in red | ||
| + | *CH5H is highlighted in light blue | ||
| + | *10x Histidine is highlighted in green | ||
| + | *B0015 is highlighted in dark blue | ||
https://static.igem.org/mediawiki/parts/8/8d/Bcht_idt.png | https://static.igem.org/mediawiki/parts/8/8d/Bcht_idt.png | ||
| − | |||
IDT report shows that the sequence is successfully synthesized: | IDT report shows that the sequence is successfully synthesized: | ||
Latest revision as of 15:27, 18 October 2016
Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator
BBa_K880005 consists of a strong promoter and strong RBS and was used to maximize protein production. This construct codes for CH25H (cholesterol 25-Hydroxylase) and a 10x Histidine-tag. CH25H is a catalyst that will make 25 hydroxycholesterol from cholesterol and 10x Histidine-tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Synthesis Report
This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below:
- Prefix and suffix are highlighted in orange
- BBa_K880005 is highlighted in red
- CH5H is highlighted in light blue
- 10x Histidine is highlighted in green
- B0015 is highlighted in dark blue
IDT report shows that the sequence is successfully synthesized:
