Difference between revisions of "Part:BBa K2074003"
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| − | [[File:2016FAFUCHINADevice1.png| | + | [[File:2016FAFUCHINADevice1.png|800px|thumb|center|'''Fig.1''' Cry4Aa+2A+Cyt1 Toxins Express Device ]] |
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Latest revision as of 21:29, 18 October 2016
Cry4Aa+2A+Cyt1 expressed in C. reinhardtii
This devise is consisted of a HSP70A-RBCS2 promoter, Cry4Aa toxin, extended FMDV 2A peptides, a Cyt1 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 251
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1641
Illegal BglII site found at 4452 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2033
Illegal NgoMIV site found at 4286
Illegal NgoMIV site found at 4586 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 686
Illegal BsaI.rc site found at 839
Illegal BsaI.rc site found at 2726
Illegal BsaI.rc site found at 3266
Illegal BsaI.rc site found at 3575
Illegal BsaI.rc site found at 4142
Illegal SapI.rc site found at 1843
Illegal SapI.rc site found at 1885