Difference between revisions of "Part:BBa K2097002"
 
(11 intermediate revisions by the same user not shown)
Line 4: Line 4:
  
 
<html>
 
<html>
−
This is a composite part made up of <a href="https://parts.igem.org/Part:BBa_K1033916">BBa_K1033916</a> (amajLime, a yellow-green chromoprotein) and <a href="https://parts.igem.org/Part:BBa_K608006"> BBa_K608006</a> (a medium promoter and RBS). It is made up of a medium promoter, <a href="https://parts.igem.org/Part:BBa_J23110"> BBa_J23110</a>, and a medium RBS, <a href="https://parts.igem.org/Part:BBa_B0032"> BBa_B0032</a>.
+
This is a composite part made up of <a href="https://parts.igem.org/Part:BBa_K1033916">BBa_K1033916</a> (amajLime, a yellow-green chromoprotein) and <a href="https://parts.igem.org/Part:BBa_K608006">BBa_K608006</a> (a medium promoter and RBS). It is made up of a medium promoter, <a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>, and a medium RBS, <a href="https://parts.igem.org/Part:BBa_B0032"> BBa_B0032</a>. Served as the control group for the part <a href="https://parts.igem.org/Parts:BBa_K2097000">BBa_K2097000</a>.
 
</html>
 
</html>
 
===Usage and Biology===
 
===Usage and Biology===
Line 12: Line 12:
 
</html>
 
</html>
  
−
[[File:YGCPtube.png|thumb|left|300px|Figure 1. amajLime expressed in <i>E. coli</i> in liquid LB]]
+
[[File:YGCPtube.png|thumb|left|275px|Figure 1. amajLime expressed in <i>E. coli</i> in liquid LB]]
−
[[File:T--Austin_UTexas--YGCPspin.png|thumb|center|350px| Figure 2. amajLime expressed in <i>E. coli</i> after being spun down in a microcentrifuge]]
+
[[File:T--Austin_UTexas--YGCPspin.png|thumb|left|300px| Figure 2. amajLime expressed in <i>E. coli</i> after being spun down in a microcentrifuge]]
  
 +
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
 
===Sequence and Features===
 
===Sequence and Features===
  
 
<partinfo>BBa_K2097002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2097002 SequenceAndFeatures</partinfo>
−
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 07:16, 18 October 2016


Yellow-green chromoprotein with medium promoter and RBS

This is a composite part made up of BBa_K1033916 (amajLime, a yellow-green chromoprotein) and BBa_K608006 (a medium promoter and RBS). It is made up of a medium promoter, BBa_J23110, and a medium RBS, BBa_B0032. Served as the control group for the part BBa_K2097000.

Usage and Biology

BBa_K1033916 was created by Uppasala 2013. It comes from the coral Anemonia majano. BBa_K608006 was created by Freiburg 2011.

Figure 1. amajLime expressed in E. coli in liquid LB
Figure 2. amajLime expressed in E. coli after being spun down in a microcentrifuge




















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]