Difference between revisions of "Part:BBa K2100046"
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<partinfo>BBa_K2100046 short</partinfo> | <partinfo>BBa_K2100046 short</partinfo> | ||
− | + | This construct is the result of a gateway combining a BM3R1 repressible promoter upstream of the mKate gene, a red fluorescent protein. pBM3R1 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The BM3R1 repressor inhibits the activator and transcription machinery from transcribing the mKate gene. | |
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Latest revision as of 05:01, 19 October 2016
pEXPR pBM3R1:mKate
This construct is the result of a gateway combining a BM3R1 repressible promoter upstream of the mKate gene, a red fluorescent protein. pBM3R1 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The BM3R1 repressor inhibits the activator and transcription machinery from transcribing the mKate gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 242
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 242
Illegal NheI site found at 115
Illegal NotI site found at 4 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 242
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 242
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 242
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 108
Illegal BsaI site found at 235
Illegal BsaI.rc site found at 906
Illegal SapI.rc site found at 288