Difference between revisions of "Part:BBa K2100045"

Latest revision as of 05:01, 19 October 2016

pEXPR pTALER14:mKate

This construct is the result of a gateway combining a TALER14 repressible promoter upstream of the mKate gene, a red fluorescent protein. pTALER14 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The TALER14 repressor inhibits the activator and transcription machinery from transcribing the mKate gene.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal BamHI site found at 303
Illegal XhoI site found at 246
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 318
Illegal BsaI.rc site found at 999
Illegal SapI.rc site found at 381

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2100045 short</partinfo>
-description here.
+This construct is the result of a gateway combining a TALER14 repressible promoter upstream of the mKate gene, a red fluorescent protein. pTALER14 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The TALER14 repressor inhibits the activator and transcription machinery from transcribing the mKate gene.
 <!-- Add more about the biology of this part here