Difference between revisions of "Part:BBa K2097002"

Latest revision as of 07:16, 18 October 2016

Yellow-green chromoprotein with medium promoter and RBS

This is a composite part made up of BBa_K1033916 (amajLime, a yellow-green chromoprotein) and BBa_K608006 (a medium promoter and RBS). It is made up of a medium promoter, BBa_J23110, and a medium RBS, BBa_B0032. Served as the control group for the part BBa_K2097000.

Usage and Biology

BBa_K1033916 was created by Uppasala 2013. It comes from the coral Anemonia majano. BBa_K608006 was created by Freiburg 2011.

Figure 1. amajLime expressed in E. coli in liquid LB

Figure 2. amajLime expressed in E. coli after being spun down in a microcentrifuge

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2097002 short</partinfo>
-This is a composite part made up of <a href="https://parts.igem.org/Part:BBa_K1033916">BBa_K1033916</a> (amajLime, a yellow-green chromoprotein)and BBa_K608006 (a medium promoter and RBS).
+<html>
+This is a composite part made up of <a href="https://parts.igem.org/Part:BBa_K1033916">BBa_K1033916</a> (amajLime, a yellow-green chromoprotein) and <a href="https://parts.igem.org/Part:BBa_K608006">BBa_K608006</a> (a medium promoter and RBS). It is made up of a medium promoter, <a href="https://parts.igem.org/Part:BBa_J23110">BBa_J23110</a>, and a medium RBS, <a href="https://parts.igem.org/Part:BBa_B0032"> BBa_B0032</a>. Served as the control group for the part <a href="https://parts.igem.org/Parts:BBa_K2097000">BBa_K2097000</a>.
-<!-- Add more about the biology of this part here
+</html>
 ===Usage and Biology===
-<!-- -->
+<html>
-<span class='h3bb'>Sequence and Features</span>
+<p><a href="https://parts.igem.org/Part:BBa_K1033916"> BBa_K1033916</a> was created by Uppasala 2013. It comes from the coral Anemonia majano. <a href="https://parts.igem.org/Part:BBa_K608006"> BBa_K608006</a> was created by Freiburg 2011.
-<partinfo>BBa_K2097002 SequenceAndFeatures</partinfo>
+</html>
+[[File:YGCPtube.png|thumb|left|275px|Figure 1. amajLime expressed in <i>E. coli</i> in liquid LB]]
+[[File:T--Austin_UTexas--YGCPspin.png|thumb|left|300px| Figure 2. amajLime expressed in <i>E. coli</i> after being spun down in a microcentrifuge]]
+<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
+===Sequence and Features===
+<partinfo>BBa_K2097002 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display