Difference between revisions of "Part:BBa K2100037:Design"

 
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===Design Notes===
 
===Design Notes===
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None
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This composite part expression vector was created by an LR reaction. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
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+
−
 
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===Source===
 
===Source===
  
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Comes from some genomic sequence.
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Comes from some mammalian genomic sequence.
  
 
===References===
 
===References===

Latest revision as of 23:20, 17 October 2016


pEXPR TRE - TALER14


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 518
    Illegal XbaI site found at 30
    Illegal XbaI site found at 495
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 518
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 518
    Illegal BamHI site found at 641
    Illegal XhoI site found at 584
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 518
    Illegal XbaI site found at 30
    Illegal XbaI site found at 495
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 518
    Illegal XbaI site found at 30
    Illegal XbaI site found at 495
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 656


Design Notes

This composite part expression vector was created by an LR reaction. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

Comes from some mammalian genomic sequence.

References