Difference between revisions of "Part:BBa K2097001"
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| − | This is a composite part made up of <a href="http://partsregistry.org/Part:BBa_K592009">BBa_K592009</a> (blue chromoprotein) and <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> is made up of a promoter, <a href="http://partsregistry.org/Part:BBa_J23104"> BBa_J23104</a>, and an RBS, <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_B0034 </a>. | + | This is a composite part made up of <a href="http://partsregistry.org/Part:BBa_K592009">BBa_K592009</a> (blue chromoprotein) and <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> is made up of a promoter, <a href="http://partsregistry.org/Part:BBa_J23104"> BBa_J23104</a>, and an RBS, <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_B0034</a>. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | BBa_K592009 was created by Uppsala-Sweden 2011. It comes from the coral Acropora millepora, amilCP. BBa_K608002 was created by Freiburg 2011. | + | <html> |
| − | + | <a href="http://partsregistry.org/Part:BBa_K592009">BBa_K592009</a> was created by Uppsala-Sweden 2011. It comes from the coral Acropora millepora, amilCP. <a href="http://partsregistry.org/Part:BBa_K608002">BBa_K608002</a> was created by Freiburg 2011. | |
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| − | [[File:T--Austin_UTexas--BCPplate.png|thumb|left|300px| Figure 1. amilCP expressed in <i> E. coli </i> ]] | + | [[File:T--Austin_UTexas--BCPplate.png|thumb|left|300px| Figure 1. amilCP expressed in <i> E. coli </i> and on an LB/agar plate ]] |
| + | [[File:T--Austin_UTexas--BCPtube.png|thumb|center|150px| Figure 2. amilCP expressed in <i> E. coli </i> and in liquid LB]] | ||
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===Sequence and Features=== | ===Sequence and Features=== | ||
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Latest revision as of 15:41, 18 October 2016
Blue chromoprotein with strong promoter and RBS
This is a composite part made up of BBa_K592009 (blue chromoprotein) and BBa_K608002 (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. BBa_K608002 is made up of a promoter, BBa_J23104, and an RBS, BBa_B0034.
Usage and Biology
BBa_K592009 was created by Uppsala-Sweden 2011. It comes from the coral Acropora millepora, amilCP. BBa_K608002 was created by Freiburg 2011.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]