Difference between revisions of "Part:BBa K1886017:Design"

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===Design Notes===
 
===Design Notes===
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Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc.
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Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. This modified PLtetO-1 promoter is designed to regulate the expression of Cph8.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455.

Latest revision as of 18:13, 17 October 2016


broken_Ptet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. This modified PLtetO-1 promoter is designed to regulate the expression of Cph8.

Source

This promoter is different from BBa_R0040. R0040 has repeated sequences of tet operator, while K1886017 has only one tet operator.This promoter is bought in Addgene company.

References

Olson E J, Hartsough L A, Landry B P, et al. Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals[J]. Nature methods, 2014, 11(4): 449-455.