Difference between revisions of "Part:BBa K2074007"

 
 
Line 4: Line 4:
  
 
This devise is consisted of a HSP70A-RBCS2 promoter, Cry11Aa toxin, extended FMDV 2A peptides, a Cyt1 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.
 
This devise is consisted of a HSP70A-RBCS2 promoter, Cry11Aa toxin, extended FMDV 2A peptides, a Cyt1 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.
−
 
+
[[File:2016FAFUCHINADevice1.png|800px|thumb|center|'''Fig.1''' Cry11Aa+2A+Cyt1 Toxins Express Device ]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 07:21, 18 October 2016


Cry11Aa+2A+Cyt1 expressed in C. reinhardtii

This devise is consisted of a HSP70A-RBCS2 promoter, Cry11Aa toxin, extended FMDV 2A peptides, a Cyt1 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.

Fig.1 Cry11Aa+2A+Cyt1 Toxins Express Device

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 251
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1172
    Illegal NgoMIV site found at 2336
    Illegal NgoMIV site found at 2684
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 704
    Illegal BsaI.rc site found at 899
    Illegal BsaI.rc site found at 2114
    Illegal BsaI.rc site found at 3203
    Illegal SapI.rc site found at 2095