Difference between revisions of "Part:BBa K2074006"

 
 
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This devise is consisted of a HSP70A-RBCS2 promoter, Cry10Aa toxin, extended FMDV 2A peptides, a Cyt2 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.
 
This devise is consisted of a HSP70A-RBCS2 promoter, Cry10Aa toxin, extended FMDV 2A peptides, a Cyt2 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.
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[[File:2016FAFUCHINADevice1.png|800px|thumb|center|'''Fig.1''' Cry10Aa+2A+Cyt2 Toxins Express Device ]]
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 07:20, 18 October 2016


Cry10Aa+2A+Cyt2 expressed in C. reinhardtii

This devise is consisted of a HSP70A-RBCS2 promoter, Cry10Aa toxin, extended FMDV 2A peptides, a Cyt2 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.

Fig.1 Cry10Aa+2A+Cyt2 Toxins Express Device

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 251
    Illegal NotI site found at 1023
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 687
    Illegal BglII site found at 2937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 878
    Illegal NgoMIV site found at 935
    Illegal NgoMIV site found at 1439
    Illegal NgoMIV site found at 2115
    Illegal NgoMIV site found at 2771
    Illegal NgoMIV site found at 3071
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 857
    Illegal SapI.rc site found at 1579