Difference between revisions of "Part:BBa K2074005"
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This devise is consisted of a HSP70A-RBCS2 promoter, Cry10Aa toxin, extended FMDV 2A peptides, a Cyt1 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii. | This devise is consisted of a HSP70A-RBCS2 promoter, Cry10Aa toxin, extended FMDV 2A peptides, a Cyt1 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii. | ||
| + | [[File:2016FAFUCHINADevice1.png|800px|thumb|center|'''Fig.1''' Cry10Aa+2A+Cyt1 Toxins Express Device ]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 07:20, 18 October 2016
Cry10Aa+2A+Cyt1 expressed in C. reinhardtii
This devise is consisted of a HSP70A-RBCS2 promoter, Cry10Aa toxin, extended FMDV 2A peptides, a Cyt1 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 251
Illegal NotI site found at 1023 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 687
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 836
Illegal NgoMIV site found at 878
Illegal NgoMIV site found at 935
Illegal NgoMIV site found at 1439
Illegal NgoMIV site found at 2115
Illegal NgoMIV site found at 2771 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 857
Illegal BsaI.rc site found at 3290
Illegal SapI.rc site found at 1579