Difference between revisions of "Part:BBa K1997011:Design"

(→‎Design Notes)
(→‎Source)
 
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===Source===
 
===Source===
  
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This part is assembled from K1789012, K1997000 and a dCas9 fragment
+
This part is assembled from K1789012, K1997005 and B0015.
−
 
+
  
 
===References===
 
===References===

Latest revision as of 04:18, 17 October 2016


P+R->sHRP-N->FRB->RBS->FKBP->sHRP-C->Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 383
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 625
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1553
    Illegal BsaI.rc site found at 908
    Illegal SapI.rc site found at 1667


Design Notes

We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced

Source

This part is assembled from K1789012, K1997005 and B0015.

References