Difference between revisions of "Part:BBa K2136013"
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<partinfo>BBa_K2136013 short</partinfo> | <partinfo>BBa_K2136013 short</partinfo> | ||
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AR1-Rbcs2 | AR1-Rbcs2 | ||
| − | Constitutive promoter for use in Chlamydomonas reinhardtii based on the fusion of DNA sequences upstream of Heat Shock Protein 70A and rbcS2 genes. | + | Constitutive promoter for use in Chlamydomonas reinhardtii based on the fusion of DNA sequences upstream of Heat Shock Protein 70A (nucleotides 1-275) and rbcS2 (nucleotides 294-474) genes. |
| + | This part also includes a 5'-UTR derived from the rbcS2 gene. | ||
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| + | ===Improvement=== | ||
| + | NU_Kazakhstan 2017 team made an improved version of this promoter by inserting high-efficiency separator between two promoters and added the first intron of RBCS2 gene. Link: [https://parts.igem.org/Part:BBa_K2516001 BBa_K2516001] | ||
Latest revision as of 21:40, 1 November 2017
hsp70 + rbsc2 synthetic constitutive promoter for Chlamydomonas
AR1-Rbcs2
Constitutive promoter for use in Chlamydomonas reinhardtii based on the fusion of DNA sequences upstream of Heat Shock Protein 70A (nucleotides 1-275) and rbcS2 (nucleotides 294-474) genes. This part also includes a 5'-UTR derived from the rbcS2 gene.
Improvement
NU_Kazakhstan 2017 team made an improved version of this promoter by inserting high-efficiency separator between two promoters and added the first intron of RBCS2 gene. Link: BBa_K2516001
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 264
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]