Difference between revisions of "Part:BBa K1936001:Design"
Mdickmanns (Talk | contribs) (→Source) |
Mdickmanns (Talk | contribs) (→Design Notes) |
||
(4 intermediate revisions by the same user not shown) | |||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | Take a look at the <html><a href="https://parts.igem.org/Part:BBa_K1936001">main page</a></html> for further information on the design of this part. | |
− | + | ||
− | + | ||
===Source=== | ===Source=== | ||
− | + | All used sequences except for linkers were codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources. | |
− | + | <html><br></html> | |
Tom5 yeast sequence:<html><br></html> | Tom5 yeast sequence:<html><br></html> | ||
http://www.ebi.ac.uk/ena/data/view/AY558369 | http://www.ebi.ac.uk/ena/data/view/AY558369 | ||
− | |||
− | |||
LOV2 sequence:<html><br></html> | LOV2 sequence:<html><br></html> | ||
from pKM291 containing LOVpep[T406A,T407A] double mutant [1] | from pKM291 containing LOVpep[T406A,T407A] double mutant [1] | ||
+ | <html><br></html> | ||
===References=== | ===References=== | ||
+ | |||
+ | <html><font size="-1">[1]</font><font size="-2"> Müller K, Engesser R, Timmer J, Zurbriggen MD, Weber W (2014) Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol. 2014 Nov 21;3(11):796-801. doi: 10.1021/sb500305v. Epub 2014 Oct 28.</font></html> |
Latest revision as of 15:25, 19 October 2016
Tom5-eGFP-LOV2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 33
Illegal BamHI site found at 712
Illegal XhoI site found at 515 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1226
Design Notes
Take a look at the main page for further information on the design of this part.
Source
All used sequences except for linkers were codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources.
Tom5 yeast sequence:
http://www.ebi.ac.uk/ena/data/view/AY558369
LOV2 sequence:
from pKM291 containing LOVpep[T406A,T407A] double mutant [1]
References
[1] Müller K, Engesser R, Timmer J, Zurbriggen MD, Weber W (2014) Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol. 2014 Nov 21;3(11):796-801. doi: 10.1021/sb500305v. Epub 2014 Oct 28.