Difference between revisions of "Part:BBa K1930002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for <em>Bacillus subtilis</em>, which can be used for cloning in <em>E. coli</em> as well. | + | The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for <em>Bacillus subtilis</em>, which can be used for cloning in <em>E. coli</em> as well. For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/Experiments |
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===Source=== | ===Source=== | ||
− | Key sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding | + | <p>Key sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding </p> |
− | BBa_K823023: Created by iGEM Munich 2012. https://parts.igem.org/Part:BBa_K823023 | + | <p> BBa_K823023: Created by iGEM Munich 2012. https://parts.igem.org/Part:BBa_K823023 </p> |
===References=== | ===References=== | ||
+ | |||
+ | [http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.''] |
Latest revision as of 10:57, 17 October 2016
Encryption key sequence in integration plasmid (BBa_K823023)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 6045
Illegal XhoI site found at 3553 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1107
Illegal NgoMIV site found at 3517
Illegal NgoMIV site found at 4656 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4480
Design Notes
The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/Experiments
Source
Key sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding
BBa_K823023: Created by iGEM Munich 2012. https://parts.igem.org/Part:BBa_K823023
References
[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury et al.]