Difference between revisions of "Part:BBa K2172002"
 
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<partinfo>BBa_K2172002 short</partinfo>
 
<partinfo>BBa_K2172002 short</partinfo>
  
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This part is a complete circuit that contains a GST label, a thrombin protease cleavage site and a GFP detector. It can be used to construct further parts that can synthesis SmCPS1.
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This part can be used to make other parts that can be purified, cleaved and detected for expression conveniently. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin restriction enzyme cleavage site and a GFP detection unit. It also has a promoter and a terminator.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:44, 19 October 2016


Tac Promoter-RBS-GST-Thrombin Protease-TEV-GFP-

This part can be used to make other parts that can be purified, cleaved and detected for expression conveniently. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin restriction enzyme cleavage site and a GFP detection unit. It also has a promoter and a terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 747
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159