Difference between revisions of "Part:BBa K2040100:Design"

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===Design Notes===
 
===Design Notes===
 +
Two illegal cutsites (XbaI and SpeI) were subsequently removed via site-directed mutagenesis.
  
 
===Source===
 
===Source===
  
−
It was isolated from the genomic DNA of our chassis organism Metarhizium anisopliae ARSEF549.
+
It was isolated from the genomic DNA of ''Metarhizium anisopliae''.
  
 
===References===
 
===References===
 +
Metarhizium anisopliae collagen-like protein Mcl1 (Mcl1) gene, complete cds.(DQ238489.1) http://www.ebi.ac.uk/ena/data/view/DQ238489
 +
 +
Metarhizium anisopliae collagen-like protein Mcl1 (Mcl1) mRNA, complete cds.(DQ238488.1)
 +
http://www.ebi.ac.uk/ena/data/view/DQ238488

Latest revision as of 18:25, 18 October 2016


PMcl1, hemolymph inducible promoter from Metarhizium anisopliae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1673
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 853
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1179
    Illegal SapI site found at 1130


Design Notes

Two illegal cutsites (XbaI and SpeI) were subsequently removed via site-directed mutagenesis.

Source

It was isolated from the genomic DNA of Metarhizium anisopliae.

References

Metarhizium anisopliae collagen-like protein Mcl1 (Mcl1) gene, complete cds.(DQ238489.1) http://www.ebi.ac.uk/ena/data/view/DQ238489

Metarhizium anisopliae collagen-like protein Mcl1 (Mcl1) mRNA, complete cds.(DQ238488.1) http://www.ebi.ac.uk/ena/data/view/DQ238488