Difference between revisions of "Part:BBa K1898100:Design"
 
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===Design Notes===
 
===Design Notes===
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We removed one point mutation from the cDNA with mutagenesis done by MissionBiotech.
 
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Primers were designed to remove the stop codon and were synthesized by Tri-I biotech.
 
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forward: 5' ATATgAATTCgCggCCgCTTCTAgATggACgTgACCATCCAgCA 3' (44)
 
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reverse: 5' ATATCTgCAgCggCCgCTACTAgTATTAggACgAgggAgCCgAg 3' (44)
 
  
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===Source===
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CH25H was ordered with one base pair changed to remove an internal cutting site and the stop codon was removed as well.
  
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cDNA of ch25h was ordered from Sino BIological Inc
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PCR primers were designed to clone CH25H into a Biobrick backbone
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forward: 5' ATATgAATTCgCggCCg 3' (17)
 +
 
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reverse: 5' ATATCTgCAgCggCC 3' (15)
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===Source===
  
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===References===
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CH25H was synthesized by IDT and the primers were synthesized by Tri-I Biotech.

Latest revision as of 11:48, 18 October 2016


ch25h, cholesterol 25 hydroxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CH25H was ordered with one base pair changed to remove an internal cutting site and the stop codon was removed as well.

PCR primers were designed to clone CH25H into a Biobrick backbone

forward: 5' ATATgAATTCgCggCCg 3' (17)

reverse: 5' ATATCTgCAgCggCC 3' (15)


Source

CH25H was synthesized by IDT and the primers were synthesized by Tri-I Biotech.