Difference between revisions of "Part:BBa K2012013"
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<partinfo>BBa_K2012013 short</partinfo>
 
<partinfo>BBa_K2012013 short</partinfo>
  
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This generator contain promotor J23101 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domain.It can generate CheZ with a high ouIt is used to detect the relationship of CheZ under different promotor strength and its swimming ability.
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This generator contains promoter J23101 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domains. Promoters with different strength are also constructed as BBa_K2012014[https://parts.igem.org/Part:BBa_K2012014],BBa_K2012016[https://parts.igem.org/Part:BBa_K2012016]
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<p>We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately<br/> the image are as following </p>
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<p>We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately.</p>
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<p>The image are as following.</p>
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<img src="https://static.igem.org/mediawiki/parts/7/7b/T--HZAU-China--CL1_control.jpg" style="width:600px;margin-left:5%;"/>
 
<img src="https://static.igem.org/mediawiki/parts/7/7b/T--HZAU-China--CL1_control.jpg" style="width:600px;margin-left:5%;"/>
 
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<p>Figure 1.<i>CL1 control was observed under light field and fluorescence field</p>.    
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<p><b>Figure 1.</b> CL1 control was observed under light field and fluorescence field.</p>.</br></br>     
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<img src="https://static.igem.org/mediawiki/parts/f/f7/T--HZAU-China--J23101-cheZ-GFP.jpg" style="width:600px;margin-left:5%;"/>
 
<img src="https://static.igem.org/mediawiki/parts/f/f7/T--HZAU-China--J23101-cheZ-GFP.jpg" style="width:600px;margin-left:5%;"/>
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<p><b>Figure 2.</b> <i>E.coli</i> with BBa_K2012013 was observed under light field and fluorescence field.</p></br></br>
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<img src="https://static.igem.org/mediawiki/parts/c/cb/T--HZAU-China--CheZ-GFP_swarming.jpg" style="width:600px;margin-left:5%;"/>
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<p><b>Figure 3.</b> The swarming plate of <i>E.coli</i> K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.</p>
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Figure 2.<i>E.coli</i> with BBa_K2012013 was observed under light field and fluorescence field;</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 15:08, 1 November 2016


Strong CheZ generator with a fusion GFP reporter

This generator contains promoter J23101 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domains. Promoters with different strength are also constructed as BBa_K2012014[1],BBa_K2012016[2]

We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately.

The image are as following.




Figure 1. CL1 control was observed under light field and fluorescence field.

.

Figure 2. E.coli with BBa_K2012013 was observed under light field and fluorescence field.



Figure 3. The swarming plate of E.coli K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1362