Difference between revisions of "Part:BBa K2065003:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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==iGEM TU Eindhoven 2016==
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how you used this part and how it worked out.
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===Applications of BBa_K2065003===
 
===Applications of BBa_K2065003===
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The construct on which mNeonGreen-CT52 was planned to be tested was succesfully created on DNA level but due to time limitations this construct was not expressed. This means mNeonGreen has not been characterized yet.  
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iGEM TU Eindhoven planned to use CT52-Caspase 9 on testing the effectivity of a homotetrameric T14-3-3 scaffold protein. Another use of CT52-Caspase 9 in our project was testing whether a T14-3-3 mediated kill switch could viable. In figure 1 a schematic representation of an measurement using CT52-Caspase 9 is depicted which could provide these results.
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[[File:T--TU-Eindhoven--Casp9As.png]]
  
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''Figure 1: the tetrameric scaffold increasing local concentrations of Caspase-9 better than a dimer, inducing the apoptosis pathway more efficiently.''
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 00:37, 19 October 2016


iGEM TU Eindhoven 2016

Applications of BBa_K2065003

iGEM TU Eindhoven planned to use CT52-Caspase 9 on testing the effectivity of a homotetrameric T14-3-3 scaffold protein. Another use of CT52-Caspase 9 in our project was testing whether a T14-3-3 mediated kill switch could viable. In figure 1 a schematic representation of an measurement using CT52-Caspase 9 is depicted which could provide these results.

T--TU-Eindhoven--Casp9As.png


Figure 1: the tetrameric scaffold increasing local concentrations of Caspase-9 better than a dimer, inducing the apoptosis pathway more efficiently.

User Reviews

UNIQ418422d609ad27ab-partinfo-00000000-QINU UNIQ418422d609ad27ab-partinfo-00000001-QINU