Difference between revisions of "Part:BBa K2042012:Design"
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===Source=== | ===Source=== | ||
| − | + | The RBS were synthesized from the genomic sequence of P.putida and the PhaC gene from Pseudomonas sp. MBEL 6-19. | |
===References=== | ===References=== | ||
Latest revision as of 21:51, 15 October 2016
RBS + PhaC_sp. MBEL 6-19
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1073
Illegal NgoMIV site found at 1352
Illegal AgeI site found at 148
Illegal AgeI site found at 461 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation.
Source
The RBS were synthesized from the genomic sequence of P.putida and the PhaC gene from Pseudomonas sp. MBEL 6-19.