Difference between revisions of "Part:BBa K2065000:Experience"

Latest revision as of 00:19, 19 October 2016

Applications of BBa_K2065000

iGEM TU Eindhoven 2016

iGEM TU Eindhoven used this part in combination with CT52-LargeBiT ("https://parts.igem.org/Part:BBa_K2065007"). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. This system was used as a read out method for T14-3-3 heterodimers. It is schemtically visualised in figure 1. For more detailed information about the application of CT52-SmallBiT visit our wiki "http://2016.igem.org/Team:TU-Eindhoven/Read-out".

Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52

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UNIQ16bc35652898e51a-partinfo-00000000-QINU UNIQ16bc35652898e51a-partinfo-00000001-QINU

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 __NOTOC__
 ===Applications of BBa_K2065000===
-This part was used in combination with LargeBiT, which dimerized on our heterodimeric T-14-3-3 scaffold proteins, and together formed the functional luciferase enzyme. The enzyme showed luminescence at 460 nm, thus confirming its working.
+==iGEM TU Eindhoven 2016==
+iGEM TU Eindhoven used this part in combination with CT52-LargeBiT ("https://parts.igem.org/Part:BBa_K2065007"). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. This system was used as a read out method for T14-3-3 heterodimers. It is schemtically visualised in figure 1. For more detailed information about the application of CT52-SmallBiT visit our wiki "http://2016.igem.org/Team:TU-Eindhoven/Read-out".
+[[File:T--TU-Eindhoven--NanolUcreadoutbb.png]]
+<br>
+''Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52''