Difference between revisions of "Part:BBa K2065000:Experience"
(→‎iGEM TU Eindhoven 2016)
 
(7 intermediate revisions by 2 users not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
−
how you used this part and how it worked out.
 
−
 
 
===Applications of BBa_K2065000===
 
===Applications of BBa_K2065000===
−
This part was used in combination with LargeBiT, which dimerized on our heterodimeric T-14-3-3 scaffold proteins, and together formed the functional luciferase enzyme. The enzyme showed luminescence at 460 nm, thus confirming its working.  
+
 
 +
==iGEM TU Eindhoven 2016==
 +
 
 +
iGEM TU Eindhoven used this part in combination with CT52-LargeBiT ("https://parts.igem.org/Part:BBa_K2065007"). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. This system was used as a read out method for T14-3-3 heterodimers. It is schemtically visualised in figure 1. For more detailed information about the application of CT52-SmallBiT visit our wiki "http://2016.igem.org/Team:TU-Eindhoven/Read-out". 
 +
 
 +
[[File:T--TU-Eindhoven--NanolUcreadoutbb.png]]
 +
<br>
 +
''Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52''
  
  

Latest revision as of 00:19, 19 October 2016


Applications of BBa_K2065000

iGEM TU Eindhoven 2016

iGEM TU Eindhoven used this part in combination with CT52-LargeBiT ("https://parts.igem.org/Part:BBa_K2065007"). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. This system was used as a read out method for T14-3-3 heterodimers. It is schemtically visualised in figure 1. For more detailed information about the application of CT52-SmallBiT visit our wiki "http://2016.igem.org/Team:TU-Eindhoven/Read-out".

T--TU-Eindhoven--NanolUcreadoutbb.png
Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52


User Reviews

UNIQ98b1cf1cb94194b9-partinfo-00000000-QINU UNIQ98b1cf1cb94194b9-partinfo-00000001-QINU