Difference between revisions of "Part:BBa K1893004"
 
(4 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1893004 short</partinfo>
 
<partinfo>BBa_K1893004 short</partinfo>
−
The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. LuxR is activated by C4-HSL. Included in the construct is the LuxR-activated promoter pLux.
 
  
−
<!-- Add more about the biology of this part here
+
The Lux receiver device consists of a constitutively expressed LuxR transcription regulator [https://parts.igem.org/Part:BBa_C0062 (BBa_C0062)] upstream of a cognate pLux promoter [https://parts.igem.org/Part:BBa_R0062 (BBa_R0062)]. LuxR is activated in the presence of a 3O-C6 AHL signal and binds to pLux to initiate transcription of a downstream coding sequence.
−
===Usage and Biology===
+
 
 +
 
 +
===Usage and biology===
 +
The Lux receiver is based on the Lux quorum-sensing system from <i>Vibrio fischerii</i>. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
 +
 
 +
In this case, the constitutively expressed LuxR transcriptional regulator [https://parts.igem.org/Part:BBa_C0062 (BBa_C0062)] is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to the LuxR-inducible promoter, pLux, to initiate transcription of a downstream gene.
 +
 
 +
 
 +
===Characterisation===
 +
We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here [https://parts.igem.org/Part:BBa_K1893005 (BBa_K1893005)]
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1893004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1893004 SequenceAndFeatures</partinfo>

Latest revision as of 03:17, 29 October 2016


Lux receiver (LuxR+pLux)

The Lux receiver device consists of a constitutively expressed LuxR transcription regulator (BBa_C0062) upstream of a cognate pLux promoter (BBa_R0062). LuxR is activated in the presence of a 3O-C6 AHL signal and binds to pLux to initiate transcription of a downstream coding sequence.


Usage and biology

The Lux receiver is based on the Lux quorum-sensing system from Vibrio fischerii. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.

In this case, the constitutively expressed LuxR transcriptional regulator (BBa_C0062) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to the LuxR-inducible promoter, pLux, to initiate transcription of a downstream gene.


Characterisation

We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here (BBa_K1893005)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985