Difference between revisions of "Part:BBa K1893002"
 
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<partinfo>BBa_K1893002 short</partinfo>
 
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The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. RhlR is activated by C4-HSL. Included in the construct is the RhlR-activated promoter pRhl.
 
  
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===Usage and Biology===
 
  
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The Rhl receiver device consists of a constitutively expressed RhlR transcription regulator [https://parts.igem.org/Part:BBa_C0171 (BBa_C0171)] upstream of a cognate pRhl promoter [https://parts.igem.org/Part:BBa_R0071 (BBa_R0071)]. RhlR is activated in the presence of a C4-AHL signal and binds to pRhl to initiate transcription of a downstream coding sequence.
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===Usage and biology===
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The Rhl receiver is based on the Rhl quorum-sensing system from <i>Pseudomonas aeruginosa</i>. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
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In this case, the constitutively expressed RhlR transcriptional regulator [https://parts.igem.org/Part:BBa_C0171 (BBa_C0171)] is activated by the binding of C4-AHL, an AHL quorum signal. The activated RhlR regulator binds to the RhlR-inducible promoter, pRhl, to initiate transcription of a downstream gene.
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===Characterisation===
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We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here [https://parts.igem.org/Part:BBa_K1893003 (BBa_K1893003)].
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1893002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1893002 SequenceAndFeatures</partinfo>

Latest revision as of 15:03, 29 October 2016


Rhl receiver (RhlR+pRhl)


The Rhl receiver device consists of a constitutively expressed RhlR transcription regulator (BBa_C0171) upstream of a cognate pRhl promoter (BBa_R0071). RhlR is activated in the presence of a C4-AHL signal and binds to pRhl to initiate transcription of a downstream coding sequence.


Usage and biology

The Rhl receiver is based on the Rhl quorum-sensing system from Pseudomonas aeruginosa. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.

In this case, the constitutively expressed RhlR transcriptional regulator (BBa_C0171) is activated by the binding of C4-AHL, an AHL quorum signal. The activated RhlR regulator binds to the RhlR-inducible promoter, pRhl, to initiate transcription of a downstream gene.


Characterisation

We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here (BBa_K1893003).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776