Difference between revisions of "Part:BBa K1899004"
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<partinfo>BBa_K1899004 short</partinfo>
 
<partinfo>BBa_K1899004 short</partinfo>
  
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<i>phlFp</i> is a constitutive promoter.
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<i>phlFp</i> - PhlF repressible promoter
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repressed by phlF repressor ([[Parts:BBa_K1725040|BBa_K1725040]])
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<br>
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induced by 2,4-Diacetylphloroglucinol ( DAPG ) inducer.  
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==Construct for characterization==
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[[File:PhlFp-E0240_2.png|thumb|500px|center|<b>Fig.1 </b> <i>phlFp</i> with reporter gene.]]
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In order to compare the promoter strength of the <i>phlFp</i> with the other two promoters, <i>tetp</i> and <i>lacp</i>, it was ligated with a GFP reporter gene, BBa_E0240, in BioBrick RFC10 standard.
  
 
===Results===
 
===Results===
 
Experiments on comparing the strength of <i>phlFp</i>, <i>tetp</i> and <i>lacp</i>, were performed. Results indicated that <i>phlFp</i> is the strongest among the three, with 1.96 times and 6 times stronger than that of <i>tetp</i> and <i>lacp</i>, respectively.
 
Experiments on comparing the strength of <i>phlFp</i>, <i>tetp</i> and <i>lacp</i>, were performed. Results indicated that <i>phlFp</i> is the strongest among the three, with 1.96 times and 6 times stronger than that of <i>tetp</i> and <i>lacp</i>, respectively.
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<!--[[File:IGEM2016 HKUST sfGFPcharacterization.jpg|thumb|600px|center|<b>Fig 1. Comparison on the strength of <i>phlFp</i>, <i>tetp</i> and <i>lacp</i>.</b> Negative control represents BBa_E0240. Characterization was done using <i>E. coli</i> strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.]]-->
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[[File:IGEM2016 HKUST constructA.png|thumb|600px|center|<b>Fig 2. Comparison on the strength of <i>phlFp</i>, <i>tetp</i> and <i>lacp</i>.</b> Negative control represents BBa_E0240. Characterization was done using <i>E. coli</i> strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<partinfo>BBa_K1899004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1899004 SequenceAndFeatures</partinfo>
  
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===TetR Orthologs Collection===
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{{Template:Collection/TetR_orthologs/Parts}}
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 15:04, 5 July 2022


phlFp promoter

phlFp - PhlF repressible promoter

Construct for characterization

Fig.1 phlFp with reporter gene.

In order to compare the promoter strength of the phlFp with the other two promoters, tetp and lacp, it was ligated with a GFP reporter gene, BBa_E0240, in BioBrick RFC10 standard.

Results

Experiments on comparing the strength of phlFp, tetp and lacp, were performed. Results indicated that phlFp is the strongest among the three, with 1.96 times and 6 times stronger than that of tetp and lacp, respectively.

Fig 2. Comparison on the strength of phlFp, tetp and lacp. Negative control represents BBa_E0240. Characterization was done using E. coli strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

TetR Orthologs Collection

Promoter CDS
pAmeR BBa_J428000 AmeR BBa_J428008
pAmtR BBa_J428001 AmtR BBa_J428009
pBetI BBa_J428053 BetI BBa_J428010
pBM3R1 BBa_J428054 BM3R1 BBa_J428045
pButR BBa_J428002 ButR BBa_J428011
pHapR BBa_J428003 HapR BBa_J428012
pHlyllR BBa_J428004 HlyllR not added
pIcaRA BBa_J428042 IcaRA BBa_J428044
pLitR not added LitR BBa_J428043
pLmrA not added LmrA BBa_J428013
Promoter CDS
pMcbR BBa_J428052 McbR BBa_J428014
pOrf2 not added Orf2 BBa_J428015
pPhlF BBa_K1899004 PhlF BBa_J428016
pPsrA BBa_J428005 PsrA BBa_J428017
pQacR BBa_J428050 QacR BBa_J428018
pScbR BBa_J428006 ScbR BBa_J428019
pSmcR BBa_J428007 SmcR not added
pSrpR BBa_J428049 SrpR BBa_J428020
pTarA BBa_J428048 TarA BBa_J428021
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165527/