Difference between revisions of "Part:BBa K2110101"
 
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<partinfo>BBa_K2110101 short</partinfo>
 
<partinfo>BBa_K2110101 short</partinfo>
  
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This is one of the site-directed mutant of the PETase gene, we change the 208th amino acid from I to V. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
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This is one of the site-directed mutant of the PETase gene, we change the 207th amino acid from S to T. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
 
Sequence and Features
 
Sequence and Features
  
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===Results===
 
===Results===
 
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
 
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
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https://static.igem.org/mediawiki/parts/thumb/7/7a/T--Tianjin--partdataI208V.png/800px-T--Tianjin--partdataI208V.png
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https://static.igem.org/mediawiki/parts/thumb/e/e9/T--Tianjin--s207tnew.png/800px-T--Tianjin--s207tnew.png
  
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We used the relative fluorescence units to represent the expression level of enzymes.
 
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https://static.igem.org/mediawiki/2016/1/1d/T--Tianjin--part-I208Vex.png/800px-T--Tianjin--part-I208Vex.png
 
  
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Finally we got the relative activities of enzymes.
 
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https://static.igem.org/mediawiki/2016/2/2f/T--Tianjin--part-I208Vbar.png/800px-T--Tianjin--part-I208Vbar.png
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 17:06, 19 October 2016


PETase site-directed mutant S207T

This is one of the site-directed mutant of the PETase gene, we change the 207th amino acid from S to T. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant. Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 79
    Illegal BglII site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Results

We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. 800px-T--Tianjin--s207tnew.png