Difference between revisions of "Part:BBa K2040101"
 
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<partinfo>BBa_K2040101 short</partinfo>
 
<partinfo>BBa_K2040101 short</partinfo>
  
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PgpdA is the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase promoter. This 2.1kb promoter containing a minimal gpdA promoter(BBa_K2040200) was isolated from vector pAN7-1(GenBank: Z32698.1).
 
  
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PgpdA is the constitutive promoter of the glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) from ''Aspergillus nidulans''. This 2.1kb promoter containing a minimal gpdA promoter([https://parts.igem.org/Part:BBa_K2040200 BBa_K2040200]) was isolated from fungal transformation vector.
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===Usage and Biology===
 
===Usage and Biology===
  
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To test the activity of gpdA promoter in entomopathogenic fungi <i>Metarhizium.anisopliae</i>, NYMU 2016 also constructed a composite part(BBa_K2040122) containing a mRFP coding region and put it downstream the promoter.
 
  
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Results indicate this gpdA promoter
 
  
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<image!>
 
  
  

Latest revision as of 11:23, 17 October 2016


Fungal promoter PgpdA


PgpdA is the constitutive promoter of the glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) from Aspergillus nidulans. This 2.1kb promoter containing a minimal gpdA promoter(BBa_K2040200) was isolated from fungal transformation vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
    Illegal XhoI site found at 693
    Illegal XhoI site found at 1075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
    Illegal AgeI site found at 1430
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109
    Illegal SapI site found at 315