Difference between revisions of "Part:BBa K2066111:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | pLacO1 controlling sfGFP expression and constitutively expressed LacI were placed onto the same Biobrick backbone flanked by UNS sequences to allow for easy cloning. This reduces the metabolic stress on the cells being transformed. | ||
===Source=== | ===Source=== | ||
| − | + | The sfGFP flourescent reporter and pLacO1 design is inspired by C. Lou, B. Stanton, Y.-J. Chen, B. Munsky, C. A. Voigt, Ribozyme-based insulator parts buffer synthetic circuits from genetic context. Nat. Biotechnol. 30, 1137 (2012). doi:10.1038/nbt.2401 pmid:23034349. The UNS sequences at the ends of the insert are derived from Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). | |
===References=== | ===References=== | ||
Latest revision as of 06:23, 29 October 2016
pLacO1 sfGFP + LacI (weak)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1087
Illegal NheI site found at 1110 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 224
Design Notes
pLacO1 controlling sfGFP expression and constitutively expressed LacI were placed onto the same Biobrick backbone flanked by UNS sequences to allow for easy cloning. This reduces the metabolic stress on the cells being transformed.
Source
The sfGFP flourescent reporter and pLacO1 design is inspired by C. Lou, B. Stanton, Y.-J. Chen, B. Munsky, C. A. Voigt, Ribozyme-based insulator parts buffer synthetic circuits from genetic context. Nat. Biotechnol. 30, 1137 (2012). doi:10.1038/nbt.2401 pmid:23034349. The UNS sequences at the ends of the insert are derived from Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013).