Difference between revisions of "Part:BBa K2100016"
 
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<partinfo>BBa_K2100016 short</partinfo>
 
<partinfo>BBa_K2100016 short</partinfo>
  
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The entry vector contains a promoter repressible by the TAL14 repressor. pTAL21 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. (Li et al., 2015) [1] A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The TAL14 repressor inhibits the activator and transcription machinery from transcribing the gene downstream of pTAL14.
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Entry vector containing the TAL14 repressor gene. TAL14 is an engineered repressor from TAL effectors, amino acids which have corresponding DNA target sites. This particular TAL gene was engineered to repress the DNA sequences found on the pTAL14 promoter.
  
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[1] Li, Y., Jiang, Y., Chen, H., Liao, W., Li, Z., Weiss, R., & Xie, Z. (2015). Modular construction of mammalian gene circuits using TALE transcriptional repressors. Nature Chemical Biology, 11(3), 207–213. http://doi.org/10.1038/nchembio.1736 Sequence and Features
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:04, 19 October 2016


pENTR Taler14

Entry vector containing the TAL14 repressor gene. TAL14 is an engineered repressor from TAL effectors, amino acids which have corresponding DNA target sites. This particular TAL gene was engineered to repress the DNA sequences found on the pTAL14 promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3305
    Illegal SpeI site found at 3248
    Illegal PstI site found at 117
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 14
    Illegal SpeI site found at 3248
    Illegal PstI site found at 117
    Illegal NotI site found at 421
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 14
    Illegal BamHI site found at 3178
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3305
    Illegal SpeI site found at 3248
    Illegal PstI site found at 117
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3305
    Illegal SpeI site found at 3248
    Illegal PstI site found at 117
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 2511
    Illegal AgeI site found at 32
  • 1000
    COMPATIBLE WITH RFC[1000]