Difference between revisions of "Part:BBa K1978002"

 
 
(6 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K1978002 short</partinfo>
 
<partinfo>BBa_K1978002 short</partinfo>
  
−
The TorA-RFP Biobrick consists of a TorA signal linked to RFP, which is fluorescent protein. The TorA signal sequence allows export of fully-folded proteins through the inner membrane via the TAT(Twin-Arginin)export system. This enables export of RFP out of the cell. The TorA sequence codes for a peptide that harbours a twin-arginine motif. This is vital for the recognition by the Tat system. Moreover, an AxA motif is present, which leads to cleavage by the leader peptidase I (Palmer & Berks 2012).
+
The TorA-RFP BioBrick consists of a TorA signal linked to RFP, which is fluorescent protein. The TorA signal sequence allows export of fully-folded proteins through the inner membrane via the TAT(Twin-Arginin Translocation) system. This enables export of RFP out of the cell. The TorA sequence codes for a peptide that harbours a twin-arginine motif. This is vital for the recognition by the Tat system. Moreover, an AxA motif is present, which leads to cleavage by the leader peptidase I (Palmer & Berks 2012).
 
MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATA
 
MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATA
  
  
  
−
<!-- Add more about the biology of this part here
+
<!-- Add more about the biology of this part here -->
−
===Usage and Biology===
+
  
 +
 +
===Usage and Biology===
 +
This BioBrick can be used to test a new organism for compatibility with BioBricks using the standart <i>torA</i> signal sequence from <i>E. coli</i>.
 
<!-- -->
 
<!-- -->
 +
 +
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1978002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1978002 SequenceAndFeatures</partinfo>

Latest revision as of 23:12, 17 October 2016


TorA-RFP

The TorA-RFP BioBrick consists of a TorA signal linked to RFP, which is fluorescent protein. The TorA signal sequence allows export of fully-folded proteins through the inner membrane via the TAT(Twin-Arginin Translocation) system. This enables export of RFP out of the cell. The TorA sequence codes for a peptide that harbours a twin-arginine motif. This is vital for the recognition by the Tat system. Moreover, an AxA motif is present, which leads to cleavage by the leader peptidase I (Palmer & Berks 2012). MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATA



Usage and Biology

This BioBrick can be used to test a new organism for compatibility with BioBricks using the standart torA signal sequence from E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 691
    Illegal AgeI site found at 803
  • 1000
    COMPATIBLE WITH RFC[1000]