Difference between revisions of "Part:BBa K2109110"

 
 
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HDVR U1A-C75T is an inducible ribozyme that cleaves in the presence of 0.2 M imidazole. It can be <i>in vitro</i> transcribed via T7-RNA Polymerase to produce a transcript of approximately ~250 bp. Ribozyme sequence obtained from (doi:10.1038) and was modified to conform to bio-brick standard.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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Latest revision as of 20:56, 27 October 2016


HDVR U1A-C75T

HDVR U1A-C75T is an inducible ribozyme that cleaves in the presence of 0.2 M imidazole. It can be in vitro transcribed via T7-RNA Polymerase to produce a transcript of approximately ~250 bp. Ribozyme sequence obtained from (doi:10.1038) and was modified to conform to bio-brick standard.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 58
    Illegal NgoMIV site found at 87
  • 1000
    COMPATIBLE WITH RFC[1000]