Difference between revisions of "Part:BBa K2180013"

 
 
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<partinfo>BBa_K2180013 short</partinfo>
 
<partinfo>BBa_K2180013 short</partinfo>
  
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This part conisist of the change of the lactose's operator by the CzrA operator ( BBa_K2180001 ) in the biobrick BBa_K2180012
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This biobrick allow the production of the blue pigment in the presence of zinc.
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<br>This is the assembly of our parts BBa_K2180001, BBa_K2180006 and BBa_K2180008
  
 
<!-- Add more about the biology of this part here
 
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[[File:T--UPMC-Paris--Seq2b.png|600px]]<br>
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The insert can also be confirmed by digestion using a unique restriction site inside the biobrick and checked by electrophoresis migration<br><br>
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2180013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2180013 SequenceAndFeatures</partinfo>

Latest revision as of 06:28, 30 October 2016


Promoter + Zinc Operator - CzrA Repressor Binding Site + Blue Pigment + Terminator

This biobrick allow the production of the blue pigment in the presence of zinc.
This is the assembly of our parts BBa_K2180001, BBa_K2180006 and BBa_K2180008

T--UPMC-Paris--Seq2b.png
The insert can also be confirmed by digestion using a unique restriction site inside the biobrick and checked by electrophoresis migration

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1039
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]