Difference between revisions of "Part:BBa K1909011"
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<partinfo>BBa_K1909011 short</partinfo> | <partinfo>BBa_K1909011 short</partinfo> | ||
− | + | Ba_K1909011 is a construct of medium Anderson promoter BBa_K608006, a strong ribosome binding site and the receptor tar-envZ, part BBa_C0082, which is a combination of the chemotaxis receptor Tar capable of sensing aspartate, and the cytosolic domain of osmosensor kinase EnvZ. | |
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+ | ===Functional Parameters=== | ||
+ | This combination has a higher expression as BBa_K1909012 and a lower expression as BBa_K1909010. | ||
+ | Testing the combination showed that the cells grew slowly (for miniprep culture more than 24 hours). | ||
+ | We assume that this combination (medium promoter and strong RBS) induces a high expression of the transmembrane receptor. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 22:51, 19 October 2016
Medium Anderson / Taz
Ba_K1909011 is a construct of medium Anderson promoter BBa_K608006, a strong ribosome binding site and the receptor tar-envZ, part BBa_C0082, which is a combination of the chemotaxis receptor Tar capable of sensing aspartate, and the cytosolic domain of osmosensor kinase EnvZ.
Functional Parameters
This combination has a higher expression as BBa_K1909012 and a lower expression as BBa_K1909010. Testing the combination showed that the cells grew slowly (for miniprep culture more than 24 hours). We assume that this combination (medium promoter and strong RBS) induces a high expression of the transmembrane receptor. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 173